Primer

Part:BBa_K5427045:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_pJump_24_RBS1_sfGFP (I GEM_2024_22)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 40


Design Notes

Forward primer for Gibson Assembly of pTac_RBS1_sfGFP in pJUMP24.


Source

Not applicable.

References